Plant Disease Diagnostics

CANARY® assays for plant pathogen detection in a variety of sample matrices

Incorporating CANARY® technology, the Zephyr Pathogen Identifier provides rapid, sensitive and specific identification of plant pathogens (bacteria, viruses, oomycetes, fungi and toxins) from leaf, stem, water and soil samples.

PathSensors has an ongoing partnership with the USDA to develop and deploy CANARY® technology to prevent harmful plant diseases from negatively impacting agriculture in the United States. As part of this effort, PathSensors is developing a high throughput instrument system, launching in 2016, to complement the rapid detection capabilities of the Zephyr.

Technology You Can Count On

The Zephyr Pathogen Identifier uses CANARY® (Cellular Analysis and Notification of Antigen Risks and Yields) technology. This novel detection technology, developed at MIT-Lincoln Laboratory. CANARY® utilizes B lymphocyte cells that have been modified to express antibodies on their cell surfaces against targets of choice, as well as to express the bioluminescent protein aequorin. Aequorin emits light when exposed to the increases in intracellular calcium levels that occur upon interaction of the biosensor’s antibody with its cognate target. Due to the rapid kinetics and signal amplification of cell-based signaling, detection of pathogens with CANARY® offers sensitivities down to 50 cfu/pfu with a 5 minute time to result. Sophisticated algorithms are used to accurately detect and identify pathogens based on the biosensor light emission.

CANARY® assays for plant disease diagnostics have been field tested at multiple USDA Plant Inspection Stations

CANARY® biosensors offer a superior level of speed and sensitivity. PathSensors has a selection of reagents available or in development for a number of plant pathogens including:

  • Ralstonia solanacearum
  • Phytophthora spp.
  • Citrus leprosis virus
  • Potyvirus spp.
  • Citrus greening disease (HLB)