Plant Disease Diagnostics

CANARY® assays take your plant health testing to a whole new level

Incorporating CANARY® technology, PathSensors provides assays for the rapid, sensitive and specific identification of plant pathogens (bacteria, viruses, oomycetes, fungi and toxins) from leaf, stem, water and soil samples.

PathSensors has an ongoing partnership with the USDA to develop and deploy CANARY® technology to prevent harmful plant diseases from negatively impacting agriculture in the United States. As part of this effort, PathSensors has developed a high throughput instrument system to complement the rapid detection capabilities of the Zephyr.

PathSensors Plant Pathogen Assays

Citrus Leprosis

Phytophthora HBL

Technology You Can Count On

The Zephyr Pathogen Identifier uses CANARY® (Cellular Analysis and Notification of Antigen Risks and Yields) technology. This novel detection technology, developed at MIT-Lincoln Laboratory. CANARY® utilizes B lymphocyte cells that have been modified to express antibodies on their cell surfaces against targets of choice, as well as to express the bioluminescent protein aequorin. Aequorin emits light when exposed to the increases in intracellular calcium levels that occur upon interaction of the biosensor’s antibody with its cognate target. Due to the rapid kinetics and signal amplification of cell-based signaling, detection of pathogens with CANARY® offers sensitivities down to 50 cfu/pfu with a 5 minute time to result. Sophisticated algorithms are used to accurately detect and identify pathogens based on the biosensor light emission.

CANARY® biosensors offer a superior level of speed and sensitivity. PathSensors has a selection of reagents available or in development for a number of plant pathogens including:

  • Ralstonia solanacearum
  • Phytophthora spp.
  • Citrus leprosis virus
  • Potyvirus spp.
  • Citrus greening disease (HLB)